Journal: Journal of visualized experiments : JoVE
Article Title: Tumorsphere derivation and treatment from primary tumor cells isolated from mouse rhabdomyosarcomas
doi: 10.3791/59897
Figure Lengend Snippet: Boscolo et al. Table of Materials
Article Snippet: Name of Material/Equipment Company Catalog Number Comments/Description DMEM/F12 Media Gibco 11320033 Component of tumosphere media DMEM high glucose media Gibco 11965092 Component of tumor cells media Ham’s F10 Media Life Technologies 11550043 Component of FACS buffer Penicillin - Streptomyocin Life Technologies 15140163 Component of tumosphere and tumor cells media Fetal Bovine Serum Omega Scientific FB-11 Component of tumor cells media Goat Serum Life Technologies 16210072 Component of FACS buffer Recombinant Human βFGF-basic Peprotech 10018B Component of tumosphere media EGF recombinant mouse protein Gibco PMG8041 Component of tumosphere media N-2 Supplements (100X) Gibco 17502048 Component of tumosphere media PBS Gibco 10010023 Component of FACS buffer and used for washing cells Fluriso (Isofluornae) anesthetic agent MWI Vet Supply 502017 Anesthetic reagent for animals Accutase cell dissociation reagent Gibco A1110501 Detach adherent cells and dissociate tumorspheres Matrigel membrane matrix Corning CB40234 Provides support to trasplanted cells Horse Serum Life Technologies 16050114 Component of cell isolation media Collagenase, Type II Life Technologies 17101015 Tissue digestion enzyme Dispase II, protease Life Technologies 17105041 Tissue digestion enzyme EDTA ThermoFisher S312500 Component of FACS buffer FxCycle™ Violet Stain Life Technologies F10347 Discriminate live and dead cells Trypan blue ThermoFisher 15250061 Discriminate live and dead cells Lipofectamine 3000 transfection reagent ThermoFisher L3000015 Transfection Reagent Recombinant mouse Flt-3 Ligand Protein R&D Systems 427-FL-005 Recombinant protein pEGFP-C1 Addgene 6084–1 GFP plasmid Celigo Nexcelom Celigo Microwell plate based image cytometer for adherent and suspension cells FACSAria II Flow Cytometry BD Biosciences 650033 Fluorescent activated cell sorter Neomycin-Polymyxin B Sulfates-Bacitracin Zinc Ophthalmic Ointment MWI Vet Supply 701008 Eyes ointment Open in a separate window Boscolo et al. Table of Materials
Techniques: Recombinant, Cell Isolation, Staining, Transfection, Plasmid Preparation, Cytometry, Flow Cytometry
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